The Power and Uniqueness of Regenerative Cells

Stem cells are a distinct kind of cells that possess the ability to replicate and regenerate themselves within the human body. Adult mesenchymal stem cells function as a kind of internal repair system, possessing the ability to differentiate to replenish other cells. These regenerative cells have the unique capacity to remain a stem cell or become another type of cell with a specific function within the human body such as bone, cartilage, muscle or skin cells. Given their powerful and unique regenerative nature, adipose derived regenerative cell therapy offers new potential in the treatment of certain indications.


Adipose Derived Regenerative Cell Therapy

Adipose derived regenerative cell therapy is a revolutionary method has proven effective in treating a number of medical conditions. It is autologous in nature, meaning that the regenerative cells used therapeutically come from the patient’s own adipose tissue.

Adipose tissue contains larger volumes of adult autologous mesenchymal stem cells which play a primary role in wound healing and tissue regeneration at the cellular level. With our protocol, these cells are easy to harvest with larger yields.

Cell Rich Fat Transfer

Cell Rich Fat Transfer (CRFT) protocol is a special harvesting process with a unique cannula, which gently removes small adipose cell clusters that have been loosened inside the body via mechanical dissociation. It is a harvesting and transfer method that yields consistent volume of highly viable adipose derived regenerative cells (ADRC’s).   The CRFT method of autologous fat transfer has been shown to be superior to other methods, including those including enzymatic processing.

The high yield of ADRC’s resulting from the in vivo dissociation of regenerative cells makes fat harvested using the CRFT protocol an ideal re-injectable material for achieving predictable outcomes. Studies have shown that CRFT protocol, which uses sterile decantation, preserves the integrity of fat cells and prevents the separation of free floating ADRC’s, versus using a centrifuge method, which tends to damage fragile cells and can deplete the separated fat of beneficial ADRC’s. Our system also adheres to the FDA’s minimal tissue manipulation guidelines and follows good tissue practice regulations, unlike other systems that use an enzyme and are used off-label.

After fat is harvested, the final product is referred to as Stromal Vascular Fraction (SVF).  While not all potential elements of SVF have yet been identified, we do know it is composed of stem cells and growth factors.  Growth factors are very powerful messengers that can signal cells in our bodies to reduce inflammation, repair, replenish and specialize for many functions and are currently being used more in clinical settings.  Another source of concentrated growth factors known as Platelet Rich Plasma (PRP) is already used frequently in orthopedics, wound care and cosmetic surgery, and performed in our office.


The Regenerative Cell procedures’ goal is to harness and enhance the body’s natural mechanism for healing. Cell Rich Fat Transfer (CRFT) is performed in-clinic by our board-certified physician and cardiovascular surgeon, Dr. Henry Childers, assisted by our credentialed medical staff. This cutting-edge procedure is normally conducted on an outpatient basis and under local anesthetic. The total procedure normally takes about 2-3 hours, varying based on the patient’s condition and overall health. The goal of regenerative cell treatment is to minimize pain and advance function without the need for invasive surgery.

This procedure essentially entails taking adipose tissue, essentially fat, out of your body, separating the regenerative cells via our CRFT protocol, and reinjecting them into injured tissue for regenerative purposes. This in-clinic treatment is completed the same day, and there is no need to ship samples to an outside laboratory and wait days for the cells to be returned for an injection on a second visit. This faster process provides increased regenerative cell counts, without manipulation.

  1. Harvesting of regenerative cells: The harvesting of regenerative cells is primarily acquired using in vivo dissociation with a liposuction procedure to obtain adipose tissue.
  2. Separation of regenerative cells: Through sterile decantation, the Stromal Vascular Fraction (SVF) is separated from the harvested fat cells.
  3. Isolation of cells: Using multi-filtration methodologies, regenerative cells and other cells are isolated from the SVF.
  4. Activation of regenerative cells: Once the isolated regenerative cells are acquired it is added to the patient’s Platelet Rich Plasma (PRP) and activated based on medical procedure protocol.

This process, during its duration, is constantly monitored and controlled by our physician. When considering regenerative cell therapy or making a decision to undergo the procedure as an ideal candidate, it is important to discuss your options with Dr. Childers. Devising a comprehensive treatment plan is crucial, as everyone is different and outcomes will vary.

Applications for Adipose Derived Regenerative Cell Therapy

Adipose Regenerative Cell Therapy is used for number medical conditions such as:

  • Arthritis Hip, Knee, Shoulder & Spinal
  • Rheumatoid Arthritis
  • Tendonitis – Shoulder, Achilles
  • Tennis Elbow
  • Golfer’s Elbow
  • Ligament Sprains
  • Bursitis
  • Degenerative Osteoarthritis
  • Osteoporosis
  • Fractures (e.g. Nonunion)
  • Cartilage Defects
  • SI Joint Inflammation or Arthritis
  • Facet Syndrome
  • Degenerative Disc Disease
  • Diabetes Mellitus – Type 1 and 2
  • COPD / Lung Disease
  • Hepatitis B and C
  • Fatty Liver, Liver Insufficiency
  • Peripheral Artery Disease

Advantages of Adipose Derived Regenerative Cell Therapy

  • Autologous in nature as the donor and recipient are the same person
  • Minimal health risk of cell rejection or disease transmission as the patient’s own cells are used
  • Higher yield of stem cells and growth factors normally obtained in a shorter period of time
  • Minimally invasive and same day outpatient procedure, making it convenient and more comfortable for patients
  • There is a much shorter time from extraction to the administration of treatment vs. bone marrow extraction
  • There is no ethical or moral controversy involved in harvesting autologous Adult Regenerative Cells (ASCs).

IV Stem Cell Therapy

Intravenous (IV) injection of mesenchymal stem cell (MSC’s) has proven effective in the treatment systemic diseases and neurological disorders. IV Stem Cell Therapy is an optional way of administering stem cell therapy via a drip methodology versus injection. Adipose tissue is normally harvested from the abdominal area and the stem cells extracted. These stem cells are combined with growth factors (PRP) from your blood and then activated. The activated stem cells are then reintroduced into the patient’s body via intravenous infusion. IV Adipose derived Stem Cell Therapy benefits can improve energy levels and enhance your immune system.

Frequently Asked Questions

  1. Why should I consider Regenerative Cell Therapy?

If you are looking for a non-surgical option with minimal risk, regenerative cell therapy potentially may be an option for you. Determinations will be dependent on your physician evaluation and health condition.

  1. What should I expect during the procedure?

Every patient is different, so outcomes will be dependent on the severity of your condition along with your body’s response to the regenerative cell therapy.

  1. What can I expect after the procedure?

As regenerative cell therapy is done on an outpatient basis, overnight hospital stays are not necessary.  After your treatment, you should be able to resume low impact activities almost immediately.

  1. When can I resume physical activity?

Dr. Childers will inform you of your post-procedure restrictions so that your treatment benefits will be maximized.

  1. Are there side effects from an adipose regenerative cell procedure?

No, as the adipose tissue used is extracted from your own body, without the introduction of foreign bodies. This minimizes the chances of rejection.


  1. Baptista, et al., An alternative method for the isolation of mesenchymal stromal cells derived from lipoaspirate samples, Cytotherapy (2009) Vol. II, No. 6, 706-‐715.
  2. Angeloni, Nutational Infrasonic Liposculpture (NIL) Adipose Derive Regenerative Cell Concentration and Viability Study Summary, non-‐published November 2011.
  3. Yoshimura, et. al, Cell-‐assisted lipotransfer for cosmetic breast augmentation: Supportive use of adipose-‐derived stem/stromal cells. Aesthetic Plastic Surgery (2008) 32: 48-‐55
  4. Conde-‐Green, Effects of centrifugation on cell composition and viability of aspirated adipose tissue processed for transplantation. Aesthetic Surgery Journal, March 2010, Vol. 30, No. 2, 249-‐255